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Thermo Fisher Premo Autophagy Tandem Sensor RFP-GFP-LC3B Kit

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For research use only; Thermo Fisher explicitly states that the kit is not for use in diagnostic procedures. Sources verified 2026-09-28: https://www.thermofisher.com/order/catalog/product/P36239 ; https://assets.thermofisher.com/TFS-Assets/LSG/manuals/MAN0026683-PremoAutophagySensors-UG.pdf

Kind
Autophagic-flux research assay
Type of test
BacMam-delivered tandem fluorescent LC3B live-cell imaging assay
Sample
Live mammalian cultured cells, including primary cells, stem cells, and neurons
Provider
Thermo Fisher Scientific
How to get
Order Invitrogen catalog P36239 from Thermo Fisher Scientific or an authorized distributor; a fluorescence microscope with GFP and RFP channels is required.
Details
The tandem LC3B sensor labels autophagosomes with acid-sensitive GFP and acid-insensitive RFP. After lysosomal fusion lowers pH, GFP is quenched while RFP persists, distinguishing dual-color autophagosomes from red autolysosomes.