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Agilent Seahorse XF Real-Time ATP Rate Assay Kit

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For research use only; Agilent states that the kit is not for use in diagnostic procedures. Sources verified 2026-09-28: https://www.agilent.com/en/product/cell-analysis/real-time-cell-metabolic-analysis/xf-assay-kits-reagents-cell-assay-media/seahorse-xf-real-time-atp-rate-assay-kit-740889 ; https://www.agilent.com/cs/library/flyers/public/5991-9233EN_ATP_rate_kit_flyer.pdf

Kind
Cellular-bioenergetics research assay
Type of test
Label-free live-cell oxygen-consumption and proton-efflux rate assay
Sample
Live cultured cells in a compatible Seahorse XF microplate
Provider
Agilent Technologies
How to get
Order catalog 103592-100 for a compatible Seahorse XFe96, XFe24, or XF96 analyzer; an XFp-format kit is sold separately.
Details
Uses oligomycin followed by rotenone and antimycin A while the analyzer measures oxygen consumption and extracellular proton efflux. Analysis reports mitochondrial, glycolytic, and total ATP production rates in real time.