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R&D Systems Recombinant E. coli Fpg FLARE Assay Kit

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For research use only. Fpg recognizes several lesion classes, so the enzyme-dependent comet signal indicates Fpg-sensitive damage rather than uniquely identifying 8-oxoguanine. Sources verified 2026-09-28: https://resources.rndsystems.com/pdfs/datasheets/4040-100-fk.pdf ; https://resources.rndsystems.com/images/site/BR_DNA%20Damage_RSDFY20-8630-web.pdf

Kind
Oxidative-DNA-lesion research assay
Type of test
Repair-enzyme-modified alkaline single-cell gel electrophoresis assay
Sample
Freshly prepared adherent or suspension cells embedded in low-melting-point agarose; validated cryopreserved cells may also be used
Provider
R&D Systems, a Bio-Techne brand
How to get
Order catalog 4040-100-FK for 75 samples; alkaline electrophoresis equipment, a fluorescence microscope and DNA stain are required.
Details
Recombinant formamidopyrimidine-DNA glycosylase excises susceptible oxidized or ring-opened bases, converting them to breaks detected as increased comet-tail migration relative to a matched buffer-only control. The kit supplies enzyme, buffer, agarose, lysis solution and FLARE slides.