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Abcam Complex I Enzyme Activity Microplate Assay Kit (Colorimetric)

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For research use only and not intended for diagnostic use. It measures NADH-dependent diaphorase activity without ubiquinone; rotenone and other inhibitors acting near the ubiquinone-binding site therefore do not inhibit this readout, so it is not a complete measure of respiratory-chain electron transfer in intact mitochondria. Sources verified 2026-09-28: https://www.abcam.com/en-us/products/assay-kits/complex-i-enzyme-activity-microplate-assay-kit-colorimetric-ab109721

Kind
Mitochondrial respiratory-complex research assay
Type of test
Antibody-capture kinetic colorimetric assay of Complex I diaphorase activity
Sample
Detergent extracts of human, rat, mouse, or bovine cultured cells, tissues, or isolated mitochondria
Provider
Abcam
How to get
Order ab109721 from Abcam; sample extraction, protein quantification, and a kinetic 450 nm absorbance microplate reader are required.
Details
Complex I is immunocaptured in coated wells. Active enzyme oxidizes NADH while reducing a supplied dye, producing an OD450 kinetic signal. The assay isolates Complex I from other sample proteins and is sensitive to enzyme amount, assembly, and regulation.