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Abcam Proteasome Activity Assay Kit

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For research use only. Measures chymotrypsin-like activity in lysates under the assay conditions; intact-cell ubiquitin-proteasome flux requires a separate experiment. Sources verified 2026-09-28: https://www.abcam.com/en-us/products/assay-kits/proteasome-activity-assay-kit-ab107921 ; https://content.abcam.com/content/dam/abcam/product/documents/107/ab107921/Proteasome-Activity-Assay-protocol-book-v9b-ab107921%20%28website%29.pdf

Kind
Proteasome-activity research assay
Type of test
Fluorometric chymotrypsin-like proteasome activity assay with inhibitor control
Sample
Mammalian cell lysate
Provider
Abcam
How to get
Order catalog ab107921 from Abcam; cell-lysate preparation and a fluorescence reader capable of approximately 350 nm excitation and 440 nm emission are required.
Details
Proteasomal cleavage of the supplied succinyl-LLVY-AMC substrate releases fluorescent AMC. Signal is compared with AMC standards and an MG-132 inhibitor control to assess chymotrypsin-like proteasome activity in cell lysates.