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OxiSelect DNA Double-Strand Break (DSB) Staining Kit

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For research use only and not for diagnostic procedures. γH2AX is a damage-response marker rather than a direct count of physical DNA breaks; the staining endpoint does not by itself measure repair-pathway choice, repair completion, or clinical genomic-instability risk. Sources verified 2026-09-28: https://www.cellbiolabs.com/dna-double-strand-break-assay ; https://www.cellbiolabs.com/sites/default/files/STA-321-dna-double-strand-break-assay.pdf

Kind
DNA-damage response research assay
Type of test
Immunofluorescence staining of histone H2A.X phosphorylated at Ser139
Sample
Fixed and permeabilized adherent mammalian cells cultured in microtiter plates
Provider
Cell Biolabs
How to get
Order catalog STA-321 from Cell Biolabs; use a fluorescence microscope or compatible fluorescence plate-imaging workflow.
Details
Cells are stained with an antibody against phospho-H2A.X Ser139 and a fluorescent secondary antibody. The included double-strand-break inducer supplies a positive control, and fluorescence reports the cellular γH2AX response associated with DNA double-strand breaks.