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Abcam Cathepsin D Activity Assay Kit (Fluorometric)

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For research use only. This semi-quantitative lysate endpoint reports substrate-cleaving activity under assay conditions rather than lysosomal cargo flux in intact cells. Sources verified 2026-09-28: https://www.abcam.com/en-us/products/assay-kits/cathepsin-d-activity-assay-kit-fluorometric-ab65302 ; https://content.abcam.com/content/dam/abcam/product/documents/65/ab65302/Cathepsin-D-Activity-assay-protocol-book-v9a-ab65302%20%28website%29.pdf

Kind
Lysosomal-protease activity research assay
Type of test
Fluorometric cleavage of an MCA-labeled cathepsin-D-preferred peptide substrate
Sample
Clarified lysates from adherent or suspension cells, or homogenized tissue extracts
Provider
Abcam
How to get
Order ab65302 from Abcam; a black 96-well plate and fluorescence reader capable of approximately 328 nm excitation and 460 nm emission are required.
Details
Cathepsin D in the lysate cleaves the MCA-labeled GKPILFFRLK(Dnp)-D-R-NH2 substrate to release fluorescence. Relative fluorescence can be normalized per million cells or per microgram of sample protein.