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Invitrogen LysoSensor Yellow/Blue DND-160

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For research use only. Uptake, loading time and experimental calibration affect interpretation; excessive accumulation can itself alkalinize organelles. This readout describes acidification rather than cargo clearance. Sources verified 2026-09-28: https://www.thermofisher.com/order/catalog/product/L7545 ; https://assets.thermofisher.com/TFS-Assets/LSG/manuals/mp07525.pdf

Kind
Acidic-organelle pH research probe
Type of test
Ratiometric dual-emission fluorescence for acidic-organelle pH comparisons
Sample
Living cultured cells loaded with the membrane-permeant dye
Provider
Thermo Fisher Scientific
How to get
Order catalog L7545 from Thermo Fisher Scientific; use fluorescence microscopy or calibrated dual-emission detection appropriate to the experimental design.
Details
The probe accumulates in acidic organelles and shifts from predominantly blue fluorescence at neutral pH toward yellow fluorescence as acidity increases. Its approximate solution pKa is 4.2; the manufacturer describes cellular measurements as qualitative or semiquantitative because the effective pKa can change in cellular environments.