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Invitrogen DQ Red BSA

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For research use only. Signal depends on substrate delivery, uptake and protease access, so intracellular results combine trafficking and proteolysis. Additional controls are needed to assess autophagic flux. Sources verified 2026-09-28: https://www.thermofisher.com/order/catalog/product/D12051 ; https://assets.thermofisher.com/TFS-Assets/LSG/manuals/mp12050.pdf

Kind
Proteolysis and lysosomal-degradation research substrate
Type of test
Self-quenched fluorogenic BSA substrate activated by proteolytic hydrolysis
Sample
Purified protease reactions or living cells and tissues after a researcher-designed loading or endocytic-uptake protocol
Provider
Thermo Fisher Scientific
How to get
Order Invitrogen DQ Red BSA catalog D12051 from Thermo Fisher Scientific; fluorescence detection and an assay-specific substrate-delivery protocol are required.
Details
BSA is labeled densely with BODIPY TR-X so intact substrate is strongly self-quenched. Proteolytic cleavage releases fluorescent peptide fragments, allowing protease activity or intracellular substrate degradation to be followed by microscopy, flow cytometry, or a fluorescence reader.