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Cayman Protein Carbonyl Colorimetric Assay Kit

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Research assay; Cayman warns that the product is not for human or veterinary use. The bulk DNPH endpoint does not identify which proteins or oxidative pathways produced the measured carbonyl groups, and the manual does not recommend low-protein urine samples. Sources verified 2026-09-28: https://www.caymanchem.com/product/10005020/protein-carbonyl-colorimetric-assay-kit ; https://cdn.caymanchem.com/cdn/insert/10005020.pdf

Kind
Oxidized-protein burden research assay
Type of test
DNPH derivatization and absorbance quantification of total protein carbonyl groups
Sample
Plasma, serum, cultured-cell lysates or tissue homogenates with sufficient protein concentration
Provider
Cayman Chemical
How to get
Order item 10005020 for up to 24 samples in duplicate. A spectrophotometric plate reader capable of 360–385 nm absorbance is required; protein normalization requires a separate protein-concentration determination.
Details
2,4-dinitrophenylhydrazine reacts with protein carbonyls to form hydrazones. Corrected hydrazone absorbance can be normalized to measured protein concentration to report carbonyl content per amount of protein.