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Cell Biolabs Hydroxyproline Assay Kit

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For research use only. Hydroxyproline provides an estimate of collagen content; collagen type, location, turnover and crosslinking require separate measurements. Sources verified 2026-09-28: https://www.cellbiolabs.com/hydroxyproline-assay-kit ; https://www.cellbiolabs.com/sites/default/files/STA-675-hydroxyproline-assay-kit.pdf

Kind
Collagen-content research assay
Type of test
Colorimetric total-hydroxyproline assay after acid hydrolysis
Sample
Acid-hydrolyzed tissue, plasma, serum, or urine
Provider
Cell Biolabs
How to get
Order catalog STA-675 from Cell Biolabs; sample hydrolysis and a 540–560 nm absorbance microplate reader are required.
Details
Hydroxyproline in hydrolyzed samples is oxidized by Chloramine T and converted to a pyrrole that forms a chromophore with Ehrlich reagent. Absorbance is compared with the supplied hydroxyproline standard curve to measure total hydroxyproline.