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Thermo Fisher MitoProbe TMRM Assay Kit for Flow Cytometry

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For research use only. Reports dye retention related to mitochondrial membrane potential. Respiration and mitochondrial mass require separate measurements. Sources verified 2026-09-28: https://www.thermofisher.com/order/catalog/product/M20036 ; https://assets.thermofisher.com/TFS-Assets/LSG/manuals/MAN0017633_MitoProbe_TMRM_Kit_for_Flow_Cytometry_UG.pdf

Kind
Mitochondrial membrane-potential research assay
Type of test
Flow-cytometric TMRM fluorescence assay with CCCP depolarization control
Sample
Living cultured mammalian cells in suspension or detached for flow cytometry
Provider
Thermo Fisher Scientific
How to get
Order catalog M20036 from Thermo Fisher Scientific; a compatible flow cytometer and live-cell preparation are required.
Details
The positively charged TMRM dye accumulates in active mitochondria according to membrane potential and produces red-orange fluorescence. Depolarization reduces retention and signal; the supplied CCCP control establishes a depolarized comparison condition.