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Dojindo Autophagic Flux Assay Kit

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Laboratory research use only, not for clinical diagnosis. Dye concentration and induction time require cell-specific optimization, and the signals track labeled compartments rather than turnover of a defined endogenous cargo. Sources verified 2026-09-28: https://dojindo.com/products/A562/ ; https://www.dojindo.com/manual/A562/

Kind
Live-cell autophagic-flux research assay
Type of test
Dual-fluorescence monitoring of autophagosomes and autolysosomes with DAPRed, DALGreen and bafilomycin A1 perturbation
Sample
Living cultured cells stained before experimental induction; the manufacturer protocol demonstrates adherent HeLa cells in dishes or multiwell slides.
Provider
Dojindo Laboratories
How to get
Order product code A562-10. Dual-dye analysis requires a fluorescence microscope with appropriate green and far-red channels; DALGreen alone can also be analyzed by flow cytometry.
Details
DAPRed labels autophagosomes and autolysosomes, while DALGreen labels autolysosomes. The supplied bafilomycin A1 inhibits lysosomal acidification, enabling condition-matched comparisons of autophagosome formation and later lysosomal processing.