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Cayman 20S Proteasome Assay Kit

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Research assay; Cayman warns that the product is not for human or veterinary use. It measures chymotrypsin-like 20S substrate cleavage in lysate, not intact-cell ubiquitin-dependent 26S proteolytic flux. Sources verified 2026-09-28: https://www.caymanchem.com/product/10008041/20s-proteasome-assay-kit ; https://cdn.caymanchem.com/cdn/insert/10008041.pdf

Kind
Proteasome catalytic-activity research assay
Type of test
Fluorometric cleavage of the chymotrypsin-like 20S proteasome substrate SUC-LLVY-AMC
Sample
Cultured-cell lysate supernatants
Provider
Cayman Chemical
How to get
Order item 10008041. The kit includes substrate, Jurkat-cell lysate positive control and EGCG inhibitor control; a fluorescence plate reader set near 360 nm excitation and 480 nm emission is required.
Details
Active 20S proteasome cleaves SUC-LLVY-AMC to release fluorescent AMC. Matched reactions with the supplied EGCG support interpretation of inhibitor-sensitive substrate cleavage. The manual also illustrates lactacystin as an additional inhibitor comparison; it is not listed among the supplied reagents.