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QuickZyme Soluble Collagen Assay

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For research use only. The assay measures soluble or solubilized triple-helical fibrillar collagen and does not distinguish individual collagen types. Sources verified 2026-09-28: https://www.quickzyme.com/product-list-assay-kits/collagen-assay/ ; https://www.quickzyme.com/wp-content/uploads/2018/03/Manual-QuickZyme-Soluble-Collagen-assay-Feb-2018.pdf

Kind
Extracellular-matrix collagen research assay
Type of test
Quantitative Sirius Red dye-binding assay for soluble or solubilized fibrillar collagen
Sample
Tissue-culture supernatants, cellular extracts, or tissue homogenates containing soluble or experimentally solubilized collagen
Provider
QuickZyme Biosciences
How to get
Order the one-plate QZBcol1-1, two-plate QZBcol1-2 or five-plate QZBcol1-5 format from QuickZyme; a 96-well reader and refrigerated plate centrifuge are required.
Details
Sirius Red binds triple-helical fibrillar collagen and the collagen-dye complex is precipitated, washed, released in alkaline solution and quantified against a collagen standard in a 96-well colorimetric format.