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R&D Systems CometAssay Single Cell Gel Electrophoresis Assay

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For research use only; the official kit instructions state that it is not for use in diagnostic procedures. Sources verified 2026-09-28: https://www.bio-techne.com/p/activity-assays/cometassay-single-cell-gel-electrophoresis-assay_4250-050-k ; https://resources.rndsystems.com/pdfs/datasheets/4250-050-k.pdf?v=20250804

Kind
DNA-damage and repair research assay
Type of test
Single-cell gel electrophoresis assay for DNA strand breaks
Sample
Suspensions of cultured or isolated cells embedded in low-melting-point agarose
Provider
R&D Systems, a Bio-Techne brand
How to get
Order catalog 4250-050-K from Bio-Techne; electrophoresis equipment, fluorescent DNA stain, and image analysis are required.
Details
Cells are immobilized in agarose, lysed, exposed to neutral or alkaline unwinding conditions, electrophoresed, and stained. Damaged DNA migrates from the nucleoid to form a comet tail whose length, DNA fraction, and tail moment quantify strand damage and can support DNA-repair studies.