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Agilent Seahorse XF Mito Fuel Flex Test Kit

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For research use only. Results are relative responses to inhibitor combinations in the selected cells and medium, so they depend on cell type, assay conditions and inhibitor sensitivity. Sources verified 2026-09-28: https://www.agilent.com/store/fr_FR/Prod-103260-100/103260-100 ; https://www.agilent.com/cs/library/usermanuals/public/XF_Mito_Fuel_Flex_Test_Kit_User_Guide%20old.pdf ; https://www.agilent.com/en/support/cell-analysis/mitochondrial-fuel-flexibility-xf-mito-fuel-flex-test

Kind
Live-cell mitochondrial substrate-use research assay
Type of test
Extracellular-flux oxygen-consumption assay with glucose, glutamine and long-chain-fatty-acid pathway inhibitors
Sample
Living adherent cells plated at an optimized density in a Seahorse XF cell-culture microplate
Provider
Agilent Technologies
How to get
Order part 103260-100 for six 24- or 96-well assays; a compatible Seahorse XF analyzer, sensor cartridge, culture plate and assay medium are required.
Details
Sequential additions of UK5099, BPTES and etomoxir perturb pyruvate, glutamine and long-chain-fatty-acid oxidation. Changes in oxygen-consumption rate are used to calculate each pathway's fuel dependency, capacity and flexibility under the assay conditions.