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Abcam Lysosomal Intracellular Activity Assay Kit

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For research use only, not for diagnostic use. The assay reports degradation of an exogenous endocytic substrate and does not by itself identify the responsible lysosomal enzyme or measure selective autophagic cargo clearance. Sources verified 2026-09-28: https://www.abcam.com/en-us/products/assay-kits/lysosomal-intracellular-activity-assay-kit-ab234622 ; https://content.abcam.com/content/dam/abcam/product/documents/234/ab234622/Lysosomal-Intracellular-Activity-Assay-protocol-book-v5a-ab234622%20(website).pdf

Kind
Endolysosomal cargo-degradation research assay
Type of test
Fluorescence dequenching of a lysosome-specific self-quenched endocytic substrate in live cultured cells
Sample
Living adherent or suspension cultured cells
Provider
Abcam
How to get
Order ab234622, formerly BioVision K448-50. Analyze substrate dequenching by fluorescence microscopy or flow cytometry; the kit supplies a self-quenched substrate, assay buffer and an experimental inhibitor control.
Details
Cells take up the proprietary self-quenched substrate as endocytic cargo. Degradation within endolysosomal vesicles releases fluorescence, and signal intensity is proportional to degradation of that substrate under the assay conditions.