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R&D Systems PARP Universal Colorimetric Assay Kit

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For research use only. The supplied-enzyme, cell-free format measures PARP catalysis and inhibition, not endogenous DNA-repair capacity in a cell or tissue sample. Sources verified 2026-09-28: https://www.rndsystems.com/products/parp-universal-colorimetric-assay-kit_4677-096-k ; https://resources.rndsystems.com/pdfs/datasheets/4677-096-k.pdf

Kind
DNA-damage-response enzyme research assay
Type of test
Cell-free colorimetric PARP-1 activity and inhibitor-screening assay on histone-coated wells
Sample
Supplied purified PARP-HSA enzyme tested with candidate inhibitors in a 96-well cell-free reaction
Provider
R&D Systems, a Bio-Techne brand
How to get
Order catalog 4677-096-K from R&D Systems; a 450 nm absorbance plate reader is required.
Details
PARP-1 uses biotinylated NAD to deposit poly(ADP-ribose) onto immobilized histones. Streptavidin-HRP and a colorimetric substrate generate absorbance proportional to catalytic activity, supporting activity curves and inhibitor IC50 measurements.